1.
Indian J Biochem Biophys
;
1989 Jun; 26(3): 136-9
Article
in English
| IMSEAR
| ID: sea-28155
ABSTRACT
Modification of leucine aminotransferase by diethylpyrocarbonate or rose bengal-sensitized photo-oxidation caused rapid inactivation of the enzyme. The inactivation of leucine aminotransferase depended on the concentration of the reagent, the time of incubation and exhibited pseudo-first order kinetics. Rose bengal-sensitized photo-oxidation was maximum at pH 6.5 and 9. Substrates leucine and alpha-ketoglutarate protected the enzyme against inactivation by these reagents, thus suggesting participation of histidine residue at the substrate binding site.
Subject(s)
Binding Sites , Diethyl Pyrocarbonate , Histidine/physiology , Indicators and Reagents , Leucine Transaminase , Plants/enzymology , Rose Bengal , Transaminases/antagonists & inhibitors
2.
Indian J Biochem Biophys
;
1972 Dec; 9(4): 310-2
Article
in English
| IMSEAR
| ID: sea-26886
Subject(s)
Animals , Binding Sites , Cyclic AMP , Dialysis , Enzyme Activation , Fructosephosphates , Glutamine , Glycosaminoglycans , Kinetics , Liver/enzymology , Rats , Tolbutamide , Transaminases/antagonists & inhibitors
3.
Indian J Exp Biol
;
1971 Jan; 9(1): 10-2
Article
in English
| IMSEAR
| ID: sea-60244
4.
Article
in English
| IMSEAR
| ID: sea-19736